After reading the article, we were surprised to see that two articles published almost contemporarily in 2003, stating that rafts contribute to the mobile lipid proton NMR signals, are never mentioned. These articles are:
1) Detergent-resistant membrane fractions contribute to the total 1H NMR-visible lipid signal in cells." Wright et al. European Journal of Biochemistry 270, 2091-2100 (2003) (article doi: 10.1046/j.1432-1033.2003.03586.x)
2) "High-resolution proton NMR measures mobile lipids associated with Triton-resistant membrane domains in haematopoietic K562 cells lacking or expressing caveolin-1." Ferretti et al. European Biophysics Journal 32, 83-95 (2003) (article doi: 10.1007/s00249-002-0273-8)
This absence is particularly evident for the article by Ferretti et al., since the similarities continue: both articles describe proton NMR measurements on K562 cells upon treatment with methyl-beta-cyclodextrin, upon extraction in Triton at 4°C, and after exposure to exogenous sphingomyelinase.
We are glad to see that the authors draw the same general conclusion: rafts are involved in the mobile lipid signal, although it is interesting to see that some of their experimental results seem diverse at first sight.
Two more references
26 February 2005
After reading the article, we were surprised to see that two articles published almost contemporarily in 2003, stating that rafts contribute to the mobile lipid proton NMR signals, are never mentioned. These articles are:
1) Detergent-resistant membrane fractions contribute to the total 1H NMR-visible lipid signal in cells." Wright et al. European Journal of Biochemistry 270, 2091-2100 (2003) (article doi: 10.1046/j.1432-1033.2003.03586.x)
2) "High-resolution proton NMR measures mobile lipids associated with Triton-resistant membrane domains in haematopoietic K562 cells lacking or expressing caveolin-1." Ferretti et al. European Biophysics Journal 32, 83-95 (2003) (article doi: 10.1007/s00249-002-0273-8)
This absence is particularly evident for the article by Ferretti et al., since the similarities continue: both articles describe proton NMR measurements on K562 cells upon treatment with methyl-beta-cyclodextrin, upon extraction in Triton at 4°C, and after exposure to exogenous sphingomyelinase.
We are glad to see that the authors draw the same general conclusion: rafts are involved in the mobile lipid signal, although it is interesting to see that some of their experimental results seem diverse at first sight.
Amalia Ferretti and Arno Knijn
Competing interests
None declared