Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts
© Nath et al; licensee BioMed Central Ltd. 2006
Received: 26 May 2006
Accepted: 09 August 2006
Published: 09 August 2006
Human papillomavirus type 16 (HPV-16) E5 protein co-operates with epidermal growth factor to stimulate mitogenesis of murine fibroblasts. Currently, little is known about which viral amino acids are involved in this process. Using sequence variants of HPV-16 E5 we have investigated their effects upon E5 transcription, cell-cycling and cell-growth of murine fibroblasts.
We demonstrate that: (i) introduction of Thr64 into the reference E5 sequence of HPV-16 abrogates mitogenic activity: both were poorly transcribed in NIH-3T3 cells; (ii) substitution of Leu44Val65 or, Thr37Leu44Val65 into the HPV-16 E5 reference backbone resulted in high transcription in NIH-3T3 cells, enhanced cell-cycle progression and high cell-growth; and, (iii) inclusion of Tyr8 into the Leu44Val65 backbone inhibited E5 induced cell-growth and repression of p21 expression, despite high transcription levels.
The effects of HPV-16 E5 variants upon mitosis help to explain why Leu44Val65 HPV-16 E5 variants are most prevalent in 'wild' pathogenic viral populations in the UK.
HPV-16 E5 acts co-operatively with epidermal growth factor (EGF) to stimulate mitosis. Whilst E5 may, or may not, bind directly to the EGF-receptor (EGFr) [8, 9], it was initially believed to impair acidification of endosomes via interaction with 16 KDa ATPase [10, 11] and thereby promote recycling of EGFr to the cell-surface . Others have suggested: that E5 perturbs EGFr trafficking from early to late endocytic structures rather than influencing acidification ; or, that E5 uses 16 KDa ATPase as a chaperone to enter the Golgi [2, 13].
We have previously described HPV-16 E5 variants which encode novel E5 protein sequences . Here we conjectured that these natural E5 protein variants may have differing mitogenic properties and that the amino acids involved in this process might be discernable. This proposal was based upon evidence that certain HPV-16 E5 variants are more prevalent than others in wild viral populations in our locality. We detected marked differences in the ability of individual HPV-16 E5 variants to induce transcription in stably transfected long-term NIH-3T3 cell lines, changes in cell-cycle profiles and cell-growth. Some variants were more mitogenic than the reference isolate of HPV-16 E5: others which were poorly mitogenic as a result of either amino acid changes or, low transcriptional efficiencies. Interestingly, those HPV-16 E5 variants most frequently detected in our local population (RFLP pattern 2) – and most commonly associated with cervical lesions – were those which had the greatest mitogenic activity in vitro.
HPV E5 constructs
HPV-16 E5 variants: Leu44Val65 (AJ244882); Thr37Leu44Val65 ( AJ44863); Thr64( AJ244840) ; Tyr8Leu44Val65, (AJ24481); the HPV-16 reference E5 sequence [25, 26] and HPV-6b E5 were amplified and cloned into pc DNA3.1Myc-His. For each HPV-16 E5 variant, a control construct, containing a TAA 'stop' codon (at codon position three) was also cloned into pc DNA3.1Myc-His.
Co-operation between HPV-16 E5 and EGF
E5 variants induce different cell-cycle profiles in the presence of EGF
Cells containing HPV-6b E5 or the HPV-16 E5 Thr64 variant resembled the 'stop' in their S-phase profiles (both p > 0.05). Increases in S-phase were high for the reference isolate (+ 17.6% c.f. the 'stop') and for the Leu44Val65 variant (+ 12.2%), but lower for Tyr8Leu44Val65 (+9.4%) and for Thr37Leu44Val65 (+ 6.3%: all p < 0.05). There were also increases in G2M-phase percentages for cells containing Leu44Val65 (+ 5.8%: p < 0.05) or Thr37Leu44Val65 (+ 2.8%: p > 0.05). Other E5 variants induced insignificant increases of G2M-phase percentages (Thr64 +1.8%; HPV-6b E5, + 1.6%; reference isolate, + 0.6%; and, Tyr8Leu44Val65, + 0.2%: all p > 0.05).
E5 variants with increased G2M-phase percentages exhibit increased cell-growth
Tyr8substitution of HPV-16 E5 is associated with high levels of p21 protein
We demonstrate that the reference isolate of HPV-16 E5 can co-operate with EGF to reduce the percentage of cells in G0G1-phase, increase those in S-phase and increase cell-growth. HPV-6b E5 exhibited similar though much less marked changes than the HPV-16 E5 reference isolate, in agreement with a previous study . In this report we have examined HPV-16 E5 variant activity under controlled conditions. Indeed, all were transcriptionally expressed under the same (T7) promoter and all had a minimal Kozak sequence  inserted around the initial ATG codon to insure equivalent translational efficiency. We have used these constructs to significantly extended previous observations observations on the biologic activity of HPV-16 E5 by analysing: HPV-16 E5 transcription in NIH-3T3 cells; cell-cycle progression; and, cell growth.
All HPV-16 E5 variants were translated in a cell-free wheat germ expression system equivalently, however, differences in E5 transcription were detected between stably transfected NIH-3T3 variant cell-lines. The HPV-16 E5 reference sequence and the Thr64 variant were transcribed at much lower levels than the other variants. Analysis of cell-growth curves indicated that those with low levels of mRNA were also those which were slower-growing (HPV-16 reference and Thr64) and had lower percentages of cells in G2M phase. In contrast for those transcribed to similar levels in NIH-3T3 cells, the Leu44Val65 and Thr37Leu44Val65 variants exhibited high growth but, this was not the case for Tyr8Leu44Val65. These data suggest that whilst the addition of Thr37 to the Leu44Val65 backbone is neutral, addition of Tyr8 is detrimental to cell-growth.
All HPV-16 E5 constructs – aside from Thr64 variant – caused a fall in the percentage of cells in G0G1-phase and an increase in S-phase, indicating that these variant E5 proteins stimulate passage through the G1/S cell-cycle checkpoint. Such an increase in the proportion of cells in S-phase may be advantageous to HPV-16, permitting it to increase the number of viral copies per cell prior to division.
The HPV-16 E5 Leu44Val65 and Thr37Leu44Val65variants, had greatest percentages of cells in G2M-phase and in greatest cell-growth. This biological activity may help explain why these particular E5 (i.e. RFLP pattern 2) variants are most prevalent (~70%) amongst wild populations of HPV-16 in inner-city London and most strongly associated with the presence of cervical lesions . In contrast, the Tyr8Leu44Val65 variant (which induced low cell growth) is an RFLP pattern 5 variant which is detected rarely amongst patients with lesions. Interestingly, the HPV-16 E5 RFLP pattern 2 HPV-16 E5 variants also co-segregate with nucleotide variation in the long control region that results in enhanced viral transcription via co-operation with the human POU transcription factor Brn3A .
The Leu44 Val65 substitutions may act to improve the structural integrity of E5 protein as both are α-helix stabilisers, whereas isoleucine (present at both sites in the reference isolate) is a helix destabiliser . However, comparison of computer-predicted transmembrane regions  of the reference and Leu44Val65 variant did not reveal significant differences (data not shown). Another possibility is that the Iso→Val65 change may enhance the activity of two putative E5 functional domains: the voltage gating motif (55Ser-x-x-Arg-x-x-x-x-x-Iso-Iso 65 -Phe-Val-x-x-Pro-x-x-Leu-x-x-x-His77: ), present in the HPV-16 E5 reference isolate and all reported mammalian connexins; and, the proposed binding site for 16 kDa ATPase (aa 54–78: ). Conversely, another E5 variant with an adjacent change at position 64 (Thr64 variant) exhibited an impoverished biological activity, confounding E5-induced mitogenesis at the G0/G1 checkpoint.
At least three EGF-independent E5 pathways exist (Figure 1), most notable being the transcriptional repression of p21 by E5 directly  or, indirectly, via E5 induction of c-jun [27, 35]. The fact that cells containing the Tyr8Leu44Val65 variant had the highest levels of p21 protein implies that the serine usually present at residue 8 may play an essential role in p21 repression. As the reference isolate and the Leu44Val65 variant respectively exhibited an intermediate and low level of p21 expression it could also be inferred that residues 44 and 65 may also be involved in p21 repression.
Accumulation of high levels of p21 protein in cells containing the Tyr8Leu44Val65 variant is also likely to effect cell-growth by up-regulating apoptosis. Indirect evidence for this was observed in the cell-growth assays: at 24 h numbers of Tyr8Leu44Val65 containing cells had fallen to 67% of the seeding concentration (100%), in contrast those containing Leu44Val65 variant increased to 137% (data not shown). These differences were even more marked at 48 h: Tyr8Leu44Val65 cells were down to 54%, whereas cells containing Leu44Val65 had nearly doubled (190%).
We also observed a reciprocal association between the levels of p21 and cyclin B1, this has been reported by others in several cell-systems and may represent a direct inhibitory effect of p21 upon cyclin-B1 biosynthesis [36, 37]. Unlike the Tyr8Leu44Val65 variant, insertion of a threonine (at position 64) into the Leu44Val65 backbone appeared to have no marked effect.
Using naturally-occurring amino acid sequence variants of HPV-16 E5 we have demonstrated that: (i) introduction of Thr64 into the reference E5 sequence abrogates mitogenic activity most probably through low levels of transcription; (ii) combined substitution of Leu44Val65 into the E5 reference backbone significantly enhances cell-cycle progression and cell-growth; (iii) addition of Thr37 to the Leu44Val65 variant had little effect upon mitogenic activity; and, (iv) inclusion of Tyr8 into the Leu44Val65 backbone severely inhibited E5 growth and E5 repression of p21 expression. These effects of HPV-16 E5 amino acid changes upon mitosis may – in part – help to explain why Leu44Val65 HPV-16 E5 variants are most prevalent in 'wild' viral populations in the UK. Thus we suggest amino acids 8 and 64 are critical for the mitogenic activity of HPV-16 E5.
HPV-16 E5 variants isolated from clinical samples: Ted (Leu44Val65, EMBL accession number AJ244882), 9785 (Thr37Leu44Val65, AJ44863) and Twp3 (Thr64, AJ244840)  were studied. Permission for the collection of clinical specimens was provided by the Research Ethics Committee of St Thomas' Hospital. In addition, E5 DNA from: HPV-16 containing CaSki cells (Tyr8Leu44Val65, AJ24481); the reference isolate of HPV-16 [25, 26]; and, from the low cancer-risk virus HPV-6b were investigated.
Construction of recombinant DNA expression vectors
HPV-16 E5 genes were amplified in polymerase chain reactions (PCR) using rTth™ DNA polymerase in two separate reactions, so that E5 open reading frames (ORF) between nucleotides (nt) 3866 and 4077 (encoding E5 amino acids 6–76) were obtained. The first PCR utilised an upstream primer (3836GGAGCTAGC TCACCATGGCAAATCTTGATA3865) which included an artificial Nhe-1 cut site (underlined) and a minimal Kozak sequence  (3847ACCATGG3853) this motif was incorporated to promote equivalent translational efficiency for all constructs and introduces an artificial alanine residue at codon position two). The second PCR used a 5' primer (3836GGAGCTAG CTCACCATGGCATAA CTTGATA3865) that also contained a 'stop' signal (underlined): both PCRs utilised the same downstream primer which encoded an artificial Bam H1 site (4110TACAGGATCC TTATG TAATTAAAAAGCGT GCATG4078). E5 PCR products were ligated into the Nhe 1 and Bam H1 sites of pc DNA3.1Myc-His (Invitrogen Ltd.). The E5 open reading frame (ORF) of HPV-6b E5 was also PCR amplified (using the upstream primer: 4103TACTATATTGTTGCTAGCCCACCATGGTGCTAA4135 and downstream, 4366TACAAATATAAAAAACGGGG ATCCCTAATTCATAT4332) and cloned using the same strategy. Plasmids were transformed into E. Coli JM109 cells and selected by ampicillin resistance. Positive colonies were screened by PCR and then sequenced to confirm the identity of the DNA inserts.
In vitro translation
A cell-free wheat-germ expression assay (Promega Ltd.) was used to determine protein translation of T7 mRNA transcripts with individual reactions supplemented with 5 μl of 35S-labelled cysteine (1 Ci/l: Amersham International Ltd.). Radiolabelled cysteine was selected since all HPV-16 E5 variants contain 4 cysteine residues. Proteins were subjected to PAGE (below) and radioactivity detected by autoradiography.
Preparation of stable NIH-3T3 cells expressing HPV E5 mRNA
NIH-3T3 cells (ATCC Ltd.) were maintained in Dulbecco's minimum essential medium supplemented with 40 mM L-glutamine, 2 × 106 U/l benzyl-penicillin, 2 g/l streptomycin sulphate (DMEM) and 10% (v/v) fetal calf serum (DMEM/FCS), in a humidified atmosphere of 5% (v/v) carbon dioxide in air. Cells were transfected with E5 containing plasmids using Lipofectin™ and grown in DMEM/FCS containing 1 g/l of G418 for 3 weeks (a dose cytotoxic for non-transfected NIH-3T3 cells within one week). Individual colonies of G418-selected cells were isolated and expanded in DMEM/FCS containing G418 to produce cell-lines for each variant. Individual cell-lines for each 'stop' construct were pooled. Variant cell-lines and the 'stop pool' were tested intermittently for mycoplasma infections: all were negative. Cell lines were tested for E5 mRNA expression using an adaption of the method described by Biswas et al. . Briefly reverse transcriptase (RT) reactions were primed using the HPV-16 E5 downstream primer (4110TACAGGATCCTTATGTA ATTAAAAAGCGTGCAT4078) with Moloney murine leukemia virus reverse transcriptase then samples were subjected to a nested PCR using internal primers located within the E5 ORF .
NIH-3T3 cells transfected with HPV-16 variants, HPV-16 'stop', or HPV-6b were seeded at 0.1 × 106 cells in 5 ml of DMEM/FCS (containing no G418) into 25 cm3 flasks. Cells were serum-starved for 24 h in DMEM, media was then replaced with fresh DMEM with (or without) recombinant human EGF (20 μg/l: Boehringer-Mannheim Ltd.) and cells incubated for a further 24 h. Bromo-deoxyuridine (BrdUrd: 10 μM in DMEM) were added and cells washed twice by centrifugation through 10 ml of Dulbecco's phosphate buffered saline (PBS) for 5 min at 200 g at room temperature (rt) and then fixed with 2 ml of ice-cold aqueuous 70% (v/v) ethanol. Fixative was removed and cells incubated in 1 ml of 0.1 M HCl containing 1 g/l pepsin for 12 min at 37°C. Reactions were halted by centrifugation (as above) through, and re-suspension in, PBS. Murine monoclonal anti-BrdUrd IgG1 heavy and kappa light chains (Becton-Dickenson Ltd: 250 μl per litre of PBS which contained 0.5% [v/v] Tween-20™ and 1% [v/v] FCS) was added for 1 h at rt, cells were washed with PBS and then incubated for 30 min at rt in the dark with fluorosceine-isothiocyanate (FITC) labelled Fab2 fragments of rabbit anti-mouse immunoglobulin (Dako Ltd.) at rt. After a further wash in PBS cells were re-suspended in, and stained with, 1 ml of propidium iodide/RNAse solution (50 g propidium iodide and 200 g RNAse/l PBS) for 15 min at rt before analysis on a Becton-Dickenson flow cytometer (FACSCalibur™). Data from 10,000 events (i.e. stained cells) were analysed (for a minimum of four times) for each reading after separation of single intact nuclei from debris and cell-clumps by gating on an FL2 area/width plot. Cells were then characterised on the basis of detection of green (FITC) and red (propidium iodide) fluorescence.
Cells were grown in 2% (v/v) in DMEM containing 2% (v/v) fetal calf serum, supplemented with EGF (20 μg/l), after seeding at a density of 0.1 × 106 cells in 20 cm3 Petri dishes. Cells were fed daily with fresh media containing EGF and cultures harvested at 24, 48 and 72 h by detachment from Petri dishes by exposure to Versene (5 ml for 2 min at 37°C). One millilitre of DMEM/FCS was added to inactivate the trypsin then cells were pelleted by centrifugation (200 g for 15 min at rt). Cell pellets were re-suspended in DMEM/FCS and viable cell numbers determined immediately by trypan-blue exclusion staining .
NIH-3T3 cells (0.1 × 106 cells in 5 ml of DMEM/FCS containing no G418) into 20 cm3 Petri dishes and left overnight. Cells were serum-starved for 24 h in DMEM, grown in DMEM supplemented with EGF (as above), then harvested over an 18 h period. Cells were lysed by suspension in RIPA buffer and cellular debris removed by centrifugation at 10,000 g for 30 sec at rt. Protein concentrations were determined using a commercial assay (BioRad Ltd.) and adjusted to enable 5 μg of total protein to be added to each well of a PAGE gel. Cellular proteins were subjected to PAGE under reducing conditions through a 4–12% Tris/glycine gradient gel (Novex Ltd.) and blotted onto Hybond-P™ membranes (Amersham International Ltd.). Each blot was next cut into strips containing the proteins of interest: cyclin B1 (60 kDa), β-actin (42 kDa) and p21 (21 kDa). Strips were blocked overnight at rt using 5% (w/v) dried milk powder (Marvel™, Premier Brands UK Ltd.) in Tris-buffered saline/Tween™ (20 mM Tris [hydroxymethyl]-amino methane; 0.2 M sodium chloride and 0.1% [v/v] Tween-20™, pH 7.5: TBS-T).
Blot strips were washed three times with TBS-T then incubated for 1 h at rt with rabbit anti-β-actin (1/1000 dilution), mouse anti-p21 (2.5 mg/l) or, mouse anti-cyclin B1 (2 mg/l: Pharminogen Ltd.) antibodies. After two washes in TBS-T strips were immersed in the appropriate horse-radish peroxidase-labelled secondary antibody (sheep anti-mouse immunoglobulin or donkey anti-rabbit immunoglobulin: Amersham International Ltd. each at a dilution of 1/250) for 1 h at rt. Bound antibody was detected using an ECL-plus™ chemiluminescence kit and ECL Hyperfilm™ (Amersham International Ltd.).
Students' t-tests were used to assist the interpretation of data.
American type culture collection
Dulbecco's minimal essential medium
epidermal growth factor
epidermal growth factor receptor
European molecular biology laboratory
Fluorescence activated cell sorting
fetal calf serum
Open reading frame
Polyacrylamide gel electrophoresis
Dulbecco's phosphate buffered saline
Polymerase chain reaction
- rtTh :
Recombinant thermostable Thermus thermophilus DNA polymerase
Tris buffered saline
We wish to thank The Richard Dimbleby Cancer Fund and of Guy's and St Thomas' Hospitals Charity for financial support. We wish to thank Ms. R. Gilchrist for expert technical assistance and advice on the analyses of cell-cycling experiments.
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