Immunoreactive localization of Gal1 in AzaC-treated BL36 cells. Cytospins were incubated with anti-Gal1 serum and peroxidase-stained (original magnification 1250×). First line: negative controls performed on untreated BL36 cells. No Gal1 was detectable with anti-Gal1 antibody. Second line: permeabilized cells treated cells stained with monospecific anti-Gal1 antibody; arrows indicate the localization of Gal1. Third line: after 144 h, Gal1 was detectable on the cell surface of nonpermeabilized cells. No immunostaining was observed with controls performed with preimmune serum (not shown).