Figure 1

Immunoblot images of nuclear cytoplasmic fractions, and gel images of reverse transcription-PCR (RT-PCR) from human mammary and colonic epithelial cell-lines. (A & C) Cropped immunoblots of nuclear and cytoplasmic extracts from breast (A) and colon (C) cell lines probed for MLL2, taspase 1, topoisomerase IIβ (control nuclear protein) and alpha-tubulin (control cytoplasmic proteins). From top, full-length MLL2 (290 kD), cleaved C-terminus fragment of MLL2 (75 kD), topoisomerase IIβ and alpha-Tubulin are cropped images from the same blot. (B & D) Total RNA was isolated from mammary (B) and colonic (D) epithelial cell lines and subjected to RT-PCR using MLL2 and GAPDH sequence specific primers spanning exon-exon junctions. Cropped images of gels showing amplified products loaded on 2% agarose gels. Top- MLL2, bottom- GAPDH. A. Nuclear and cytoplasmic fractions from mammary epithelial cell lines from a single blot. B. MLL2 transcript from mammary epithelial cell lines from a single gel. C. Nuclear and cytoplasmic fractions from colonic epithelial cell lines from a single blot. D. MLL2 transcript from colonic epithelial cell lines from a single gel.