Figure 3
Representative immunohistochemistry staining for MLL2 in human FFPE colonic tissue sections. A. Note differential cytoplasmic MLL2 immunostaining in tissue section from invasive carcinoma of the colon (solid arrow) and adjacent normal mucosa (hollow arrow), ×400. B. Nuclear immunoreactivity of MLL2 (arrow) in invasive colon carcinoma, ×400 C. Weakly positive MLL2 immunostaining in adjacent benign colonic epithelial cells (arrows), ×200 from same patient whose tumor sample is shown in (B) D. Anti-MLL2 antibody was incubated with blocking peptide and added to the slide with FFPE colon tissue, followed by washing and staining with secondary antibody and visualization by using diaminobenzidine (DAB) as a precipitating enzyme product.