Epigenetic regulator MLL2 shows altered expression in cancer cell lines and tumors from human breast and colon

Table 1 Relative levels of MLL2 protein in mammary epithelial cell lines

Cell lines	Origin, Description, ER status	Nuclear MLL2		Cytoplasmic MLL2
		75 kD	290 kD	75 kD	290 kD
		(Cleaved)	(Uncleaved)	(Cleaved)	(Uncleaved)
184A1	Normal breast epithelium, immortalized, ER -	+	-	+	-
MCF10a	Normal breast epithelium, immortalized, ER-	+++	-	++	-
T47D	Early stage breast carcinoma, tumorigenic, non-metastatic, ER +	++	-	+++	-
MCF7	Early stage breast carcinoma, tumorigenic, non-metastatic, ER +	++	-	+	+ +
MDA-MB-157	Advanced breast carcinoma, Tumorigenic and metastatic, ER -	+	+ + ++	+++	+ + +
MDA-MB-231	Advanced breast carcinoma, Tumorigenic and metastatic, ER -	+	+ + ++	+++	+ + +

Distribution of cleaved and uncleaved MLL2 protein in nuclear and cytoplasmic fractions of normal and tumor derived mammary epithelial cell lines
Staining intensity - Negative; + Faint; ++ Weak; +++ Moderate, ++++ Intense

ISSN: 1475-2867