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Figure 1 | Cancer Cell International

Figure 1

From: A natural compound from Hydnophytum formicarium induces apoptosis of MCF-7 cells via up-regulation of Bax

Figure 1

DNA fragmentation analysis of MCF-7 cells treated with 3HFD. Results of the DNA fragmentation analysis are presented as follows: untreated (lane 2); treated with 15 μg/ml of 3HFD for 72 h (lane 3); treated with 9 μg/ml of 3HFD for 12 h (lane 4), 24 h (lane 5), 48 h (lane 6) or 72 h (lane 7). Comparison with 100 bp (lane 1) and 1 kb (lane 8) molecular markers from Promega™ showed that lanes 4-7 were repeated fragments at 180 and 360 bp, whereas lane 3 exhibited a different fragmentation pattern with no bands corresponding to either 360 bp or 2400 bp were observed. This banding pattern may be explained by the detachment of the cells from the monolayer after 72 hours of incubation, because the cells lost the small fragment and the bigger fragment was not yet generated. The 180 bp fragments were weakly detected.

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