Figure 2
From: MicroRNA-26a-mediated regulation of interleukin-2 expression in transformed avian lymphocyte lines
Chicken IL-2 is a target for miR-26a. (a) Nucleotide sequences of the wild-type and mutant miR-26a binding sites (mutated residues underlined) located in the 3' UTR of Gallus gallus IL-2 gene (NM_204153). (b) Reporter assays on DF-1 cells transfected with the reporter vectors containing either the wildtype or mutated IL-2 3' UTR. Reduction in the ratio of Renilla to Firefly luciferase levels (p < 0.05) in the wildtype construct to the normalised 100 per cent levels for the mutant construct is shown.