Kaempferol and/or cisplatin's effect on ABCC1, ABCC5, ABCC6, NFκB1, cMyc and CDKN1A mRNA levels. OVCAR-3 cells (5 × 105) were seeded in 60-mm cell culture dishes and incubated for 16 hours before chemical treatment (0, 40, and 80-μM cisplatin with or without 20-μM kaempferol) for another 24 hours. RNA was extracted with TRIzol Reagent and quantitated by qRT-PCR as described in Materials and Methods. Gene mRNA levels were adjusted by GAPDH and expressed as percent of control. Data represents MEAN ± SE from 3 independent experiments. * p < 0.05 as compared to control by ANOVA Dunnett t test. ** p < 0.01 as compared to control by ANOVA Dunnett t test.