Figure 3

Imaging of pharmacodynamics of PI3K/AKT-kinase inhibitors in live animals [57]. (A) Mice transplanted with D54 cells stably expressing bioluminescent Akt reporter (BAR) were treated with vehicle control (20% DMSO in PBS), API-2 (20 mg/kg or 40 mg/kg) or perifosine (30 mg/kg). Images of representative mice are shown before treatment, during maximal luciferase signal upon treatment (Max), and after treatment. (B) Tumor-specific bioluminescence activity of D54 cells stably expressing BAR, treated with either the vehicle control (20% DMSO in PBS) or API-2 (20 mg/kg or 40 mg/kg), was monitored at various times. Fold induction of signal intensity over pretreatment values was plotted as mean ± s.e.m. for each of the groups. (C) Bioluminescence activity in tumor-bearing mice before treatment and in response to treatment with 30 mg/kg perifosine, plotted as fold induction over pretreatment values (± s.e.m.) for each of the groups.