VEGF levels in culture media of MDA-MB-231 cells and in vitro angiogenesis following various treatments. (A): Tissue culture media from parental MDA-MB-231 cells were collected for VEGF measurements by ELISA; bars represent means + SD of four independent experiments (n = 4). (B): Panels A-C: BCE cells co-cultured with HepG2 cells under normoxia; hypoxia; or hypoxia with 50 μM FAC. Panels D-F: BCE cells co-cultured with MDA-MB-231 cells under normoxia; hypoxia; hypoxia with 50 μM FAC. Panels G-I: BCE cells alone under normoxia; hypoxia; or normoxia with basic fibroblast growth factor (2 ng/ml). * Significantly different from normoxic controls (p < 0.05). +Significantly different from hypoxia alone (p < 0.05).