Figure 6

Further results (continuation of Figure 5) of the Western immunoblot analysis. Same as in Figure 5, except that Western immunoblot assays of the cells were performed using antibodies against (a) total Akt/PKB, (b) Akt/PKB phosphorylated at Thr308, (c) total IRS-1 (insulin receptor substrate 1), IRS-1 phosphorylated at Ser636/639, total PTEN (phosphatase and tensin homolog), PTEN phosphorylated at Ser380, total eIF4E (eukaryotic translation initiation factor 4E), eIF4E phosphorylated at Ser209, total eIF2α (eukaryotic translation initiation factor 2α), and eIF2α phosphorylated at Ser52, (d) PDGFRβ (platelet-derived growth factor receptor b) phosphorylated at Tyr751, and (e) p44/42 MAPK or ERK1/2 phosphorylated at Thr202Tyr204. All assays were performed in triplicates and repeated three times.