Effects of PHR, HC, EGF, E2, and 2-Fl-E2 on the appearance of transformed foci in MCF-10A cells after 5 weeks in continuous culture. MCF-10A cells, plated at 5 × 105 cells/well, were exposed to different culture and treatment conditions for 5 weeks in continuous culture. Cells were maintained and treated once a week in 6-well TC plates. The average number of transformed foci/well that appeared at 5 weeks in triplicate wells of each culture/treatment type from one experiment was used for analysis. Significance of differences was analyzed using One-way ANOVA followed by either Dunnet's or Tukey's test.
Using One-way ANOVA followed by Dunnet's test:
-HC/-EGF/-PHR/E2 vs -HC/-EGF/-PHR/EtOH **p < 0.0005
-HC/-EGF/-PHR vs -HC/-EGF/-PHR/EtOH *p < 0.05
Using One-way ANOVA followed by Tukey's test:
-HC/-EGF/-PHR/E2 vs all groups Φp < 0.0005
-HC/-EGF/-PHR vs all groups except -HC/-EGF/-PHR/EtOH #p < 0.0005