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Table 1 Effects of PHR, HC, EGF, E2, and 2-Fl-E2 on the Appearance of Transformed Foci in MCF-10A Cells after 5 Weeks in Continuous Culture

From: Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors

Treatments Number of Foci/Well
(+HC/+EGF) +PHR 0.00
(+HC/+EGF) -PHR 0.00
(+HC/+EGF) -PHR; 1 nM E2 0.00
(-HC/-EGF) +PHR 0.00
(-HC/-EGF) -PHR 79.50 +/- 6.50*, #
(-HC/-EGF) -PHR; 0.01% EtOH 33.50 +/- 27.50
(-HC/-EGF) -PHR; 1 nM E2 182.00 +/- 2.00**
(-HC/-EGF) -PHR; 1 nM 2-Fl-E2 19.00 +/- 5.00*
(-HC/+EGF) +PHR 0.00
(-HC/+EGF) -PHR 0.00
(-HC/+EGF) -PHR; 1 nM E2 0.00
(+HC/-EGF) +PHR 0.00
(+HC/-EGF) -PHR 0.00
(+HC/-EGF) -PHR; 1 nM E2 0.00
  1. Effects of PHR, HC, EGF, E2, and 2-Fl-E2 on the appearance of transformed foci in MCF-10A cells after 5 weeks in continuous culture. MCF-10A cells, plated at 5 × 105 cells/well, were exposed to different culture and treatment conditions for 5 weeks in continuous culture. Cells were maintained and treated once a week in 6-well TC plates. The average number of transformed foci/well that appeared at 5 weeks in triplicate wells of each culture/treatment type from one experiment was used for analysis. Significance of differences was analyzed using One-way ANOVA followed by either Dunnet's or Tukey's test.
  2. Using One-way ANOVA followed by Dunnet's test:
  3. -HC/-EGF/-PHR/E2 vs -HC/-EGF/-PHR/EtOH **p < 0.0005
  4. -HC/-EGF/-PHR vs -HC/-EGF/-PHR/EtOH *p < 0.05
  5. Using One-way ANOVA followed by Tukey's test:
  6. -HC/-EGF/-PHR/E2 vs all groups Φp < 0.0005
  7. -HC/-EGF/-PHR vs all groups except -HC/-EGF/-PHR/EtOH #p < 0.0005