Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors

Table 3 Estrogen Receptor Gene Expression in MCF-10A Cells Under Various Treatment and Culture Conditions

Group	Culture/Treatment Conditions	ERα (Mean Gene Expression)	ERβ (Mean Gene Expression)
1	+HC/+EGF; NT	62.75	9.86
	-HC/+EGF (#21); NT	57.98	5.88
2	+HC/+EGF; NT	59.79	15.82
	-HC/-EGF (#1); 1 nM E2	57.48	10.21
3	-HC/-EGF (#1); NT	50.77	a
	-HC/-EGF (#1); 1 nM E2	53.96	a

ER gene expression in MCF-10A cells maintained in and exposed to various culture/treatment conditions was assessed using a human estrogen signaling pathway-specific gene expression profiling system (SuperArray, Inc; Bethesda, MD) as described in the Methods section. Results shown are those of three different experiments comparing two different culture conditions at a time and using 5-10 μg total RNA. MCF-10A cells grown in culture media either containing or lacking HC/EGF were either left non-treated (NT) or treated twice a week with 1 nM E2 for 2 weeks. Numbers in parentheses indicate the number of passages in that particular medium type. Mean signal intensity (pixels × 10^-4) values from duplicate spots of both ERα and ERβ per membrane were normalized against those of a housekeeping gene and taken to be representative of mean gene expression.
a) Signal too weak to evaluate

ISSN: 1475-2867