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Table 5 Comparison of Estrogen Signaling Pathway Gene Expression in MCF-10A - HC/+EGF (#21) versus +HC/+EGF Cells

From: Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors

Group Gene Mean Gene Expression in +HC/+EGF Mean Gene Expression in -HC/+EGF #21
1 c-fos 22.50 +/- 4.70 12.36 +/- 3.37
2 c-jun 11.87 +/- 2.78 3.83 +/- 2.20
3 c-myc 2.20 +/- 0.03 0.00 +/- 0.00**
4 EBAG9 8.11 +/- 3.2 1.82 +/- 0.79
5 EGF 8.05 +/- 2.98 2.08 +/- 0.25
6 H-ras 6.75 +/- 1.35 1.95 +/- 1.85
7 PRL 32.19 +/- 2.42 8.57 +/- 0.16*
8 pS2 1.16 +/- 0.91 3.73 +/- 1.84
  1. MCF-10A cells maintained in +HC/+EGF medium were either kept in this medium or subsequently grown for 21 passages in -HC/+EGF medium. Gene expression analysis of genes involved in the estrogen signaling pathway in MCF-10A cells grown in these two media types were compared using a gene expression profiling system (SuperArray, Inc; Bethesda, MD) as described in the Methods section. Mean signal intensity (Pixels × 10-4) values from duplicate spots of each cDNA per membrane were normalized against those of a housekeeping gene and taken to be representative of mean gene expression. Results shown are those of one experiment comparing two different culture conditions. Significance of differences between expression of each gene in MCF-10A -HC/+EGF #21 cultures versus expression of its respective control in MCF-10A +HC/+EGF cultures was analyzed using one-tailed Student's "t" test assuming unequal variances. *p < 0.05, **p < 0.005