Figure 4From: Activating Transcription Factor 3 regulates in part the enhanced tumour cell cytotoxicity of the histone deacetylase inhibitor M344 and cisplatin in combinationM344 induced ATF3 is mediated through the Integrated Stress Response. A, Western blot analysis for ATF3 and actin in ATF4 (+/-) and (-/-) MEFs treated with solvent control, thapsigargin (1 μM), M344 (1 and 5 μM) for 24 hrs. B, Chromatin Immunoprecipication (ChIP) assay of chromatin/DNA complexes isolated from MCF-7 cells treated with solvent control or M344 (5 μM) for 24 hrs using acetylated Histone 4 (AcH4) antibody and analyzing by PCR for presence of ATF3 or p21 promoter regions.Back to article page