Figure 5From: Activating Transcription Factor 3 regulates in part the enhanced tumour cell cytotoxicity of the histone deacetylase inhibitor M344 and cisplatin in combinationATF3 mediates, in part, the enhanced cytotoxicity between M344 and cisplatin. A, A549 cells treated with cisplatin alone (0-10 μg/ml), or with M344 (1 μM) for 48 hrs were assessed for cell viability as measured by MTT activity. Data is represented as a percentage of MTT activity where untreated cells were taken to be 100%. Error bars are representative of six replicates of two independent experiments. B, A549 cells treated with solvent control, M344 alone (1 and 5 μM), cisplatin (2 μg/ml) alone or in combination with M344 (1 and 5 μM) were analyzed by Western blotting for ATF3, PARP and actin.Back to article page