Figure 6

Targeting ATF3 expression inhibits M344 and cisplatin co-operative cytotoxicity. A, A549 cells stably expressing GFP or shATF3-1 and shATF3-2 were treated with cisplatin (1-5 μg/ml) for 48 hrs and analysed for MTT activity as above. B, A549 cells stably expressing GFP or shATF3-1 and shATF3-2 were treated with cisplatin in the presence of M344 (1 μM) for 48 hrs and analysed for MTT activity as above. * Statistical analysis, Paired T-test P < 0.005 between GFP and both shATF3 sub-lines. C, A549 cells stably expressing GFP (negative control), or short hairpin RNA against two separate ATF3 mRNA regions (shATF3-1) and (shATF3-2) were untreated, treated with cisplatin (10 μg/ml) or cisplatin in combination with M344 (5 μM) for 24 hrs and analyzed by Western blotting for ATF3 and actin expression.