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Table 1 Ngb, Cygb and CA IX expression in tissue microarrays of human solid tumors and adjacent normal tissues

From: Hypoxic regulation of cytoglobin and neuroglobin expression in human normal and tumor tissues

Organ NGB CGB CAIX Comments
Normal     
Skin 1 1 0 Cygb: + desquamating cells
Breast 1 1 0 Ngb & Cygb: + ductal cells
Spleen 2 1 0  
Lymph node 3 1 1 Ngb & Cygb: + white pulp, lymph
Skeletal muscle 0 0 0  
Lung 0 0 0  
Salivary gland 2 2 1 Ngb & Cygb: + ductal cells
Liver 3 2 1  
Gallbladder 2 1 1  
Pancreas 3 0 0  
Tonsil 2 1 1  
Esophagus 2 0 1  
Stomach, antrum 2 0 1 Ngb & Cygb: + ductal cells
Stomach, fundus 3 2 2 Ngb & Cygb: + ductal cells
Small bowel 3 1 2 Ngb & Cygb: + ductal cells
Kidney, cortex 3 2 1 Ngb & Cygb: + collecting ducts & tubules; - glomeruli
Kidney, medulla 3 2 1 Ngb & Cygb: + collecting ducts & tubules; - glomeruli
Urinary bladder 3 1 1  
Prostate 4 1 1  
Testis 1 0 0 Ngb & Cygb: + ductal cells
Uterine cervix 1 1 0 Ngb & Cygb: + desquamating cells, - basal layer
Endometrium 2 0 0 Ngb & Cygb: + ductal cells
Myometrium 2 1 1  
Placenta 1 0 0 Ngb & Cygb: + ductal cells
Adrenal gland NE 2 0 Cygb: + ductal cells
Thyroid NE NE NE  
Cerebrum 3 3 1  
Cerebellum 2 2 1  
Tumor     
Skin 1 1 1  
Skin NE NE NE Ngb & Cygb: indistinguishable from melanin
Subcutis 1 0 0  
Breast 4 1 0 Ngb & Cygb: + ductal cells
Breast 3 1 2 Ngb & Cygb: + ductal cells
Hodgkins     
lymphoma 3 2 0  
Bone 5 0 0  
Lung 3 0 0  
Lung 2 1 1  
Liver 4 2 0  
Liver 4 3 2  
Liver 5 2 2  
Esophagus 2 0 1  
Stomach 1   0  
Stomach 3   1  
Stomach 3   0  
Duodenum 1 ?? 0  
Sigmoid colon 4   0  
Rectum 4   1 Ngb: + ductal cells
Kidney 4   0 Ngb: + villi, - stroma; Cygb:+ normal & tumor cells
Urinary bladder 1   3 Ngb: + infiltrating cells
Prostate 3   1 Ngb: + ductal cells
Testis 2   0 Ngb: + ductal cells
Uterine cervix 2   1  
Endometrium 1   1 Ngb: some + ductal cells
Ovary 2   0  
Ovary 3   0  
Ovary 1   1  
Thyroid 2   3  
  1. Tissue microarrays constructed from cores obtained from various human solid tumors and normal adjacent tissues were purchased from IMGENEX Corporation (San Diego, CA). Immunopositive staining for Ngb, Cygb and CA IX was assessed by two observers who noted the overall tissue staining and the presence of positively stained focal regions. Each section was assigned an overall score, where 0 = absence of positively stained cells; 1 = a few positive cells/regions ( < 10%); 2 = weak positive staining, occasional positive foci; 3 = intermediate staining, occasional positive foci ( < 50%); 4 = strongly positive in most of the section, several positive foci; and 5 = strongly positive throughout with many intensely positive foci. Distinct features of the immunostaining patterns in individual tissue cores are noted in the comments column, where "+" = positive staining, "-" = negative staining and NE = not evaluable.