Attenuating L1CAM in MDA-MB-231 decreases cell adhesion and migration. Efficiency of L1CAM-shRNA to knock down L1 expression in MDA-MB-231 cells was examined by rtPCR (A) and western blot (B). (C) Analysis of cell adhesion after L1-shRNA infection. Assays were done as previously described. (D) Cell transmigration assay was done as described previously on laminin- or fibronectin-coated transwell inserts. Results shown are mean ± SEM of three independent experiments with triplicates. **, P < 0.01 vs MDA-MB-231 cells infected with control vector.