Mechanisms of bradykinin effects on ATP resensitization. Graph A shows the average (± SEM) of ratio of the peak Ca2+ response and graph B shows the average (± SEM) ratio of the area under the curve (AUC) of the Ca2+ response of the second ATP response for all conditions tested. The ratio of the peak Δ/Fo (A) or AUC (B) for the 2nd vs. 1st response to ATP was designated as the % resensitization for each cell, and these values were averaged for all cells. Statistical significance was obtained using one way ANOVA with post-hoc Turkey. ***p < 0.001, **p < 0.01 and *p < 0.05, denotes statistic significance. Note that the enhancement of resensitization persisted on removal of extracellular Ca2+, but is lost on inhibition of phosphatases with okadaic acid, and by inhibition of PI3 kinase with LY294002.