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Figure 4 | Cancer Cell International

Figure 4

From: α-TEA-induced death receptor dependent apoptosis involves activation of acid sphingomyelinase and elevated ceramide-enriched cell surface membranes

Figure 4

α-TEA induces ASMase activation. A, ASMase activity was detected using the Acid Sphingomyelinase Assay kit (Echelon) at different time points following treatment with 40 μM α-TEA. B, Cellular localization of ASMase following treatment with 40 μM α-TEA for 4 and 9 hrs (9 hrs vehicle treatment) was detected (green channel) using immunofluorescent staining of paraformaldehyde fixed cells. Arrows in α-TEA treated cells point to membrane ASMase versus arrows in vehicle control point to cellular dispersion of ASMase.

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