Skip to main content

Table 1 Primers involved in RT-PCR reaction.

From: Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells

Genes

Primers

Sequence

Expected Product Size (bp)

Gapdh

Forward

5' CACTCCAATCGTCCCTACA 3'

 
 

Reverse

5' AAGGTGGAAGAGTGGGAG 3'

717

Opn

Forward

5' CACTCCAATCGTCCCTACA 3'

 
 

Reverse

5' GCTGCCCTTTCCGTTGTT 3'

234

CatK

Forward

5'GGCAGGGTCCCAGACTCCAT3'

 
 

Reverse

5'GTGTTGGTGGTGGGCTAC3'

350

Sca-1

Forward

5'GGCAGGGTCCCAGACTCCAT3'

 
 

Reverse

5'GTGTTGGTGGTGGGCTAC3'

167

  1. Gapdh; glyceraldehyde-3-phosphate dehydrogenase as housekeeping gene, Opn; Osteopontin as osteoblast marker, CatK; Catepsin K as osteoclast marker, Sca-1; Stem cell antigen-1 as murine hematopoietic stem cell marker.