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Table 3 Methylation specific primer (MSP) pairs.

From: Loss of glutathione peroxidase 3 expression is correlated with epigenetic mechanisms in endometrial adenocarcinoma

Primer pairs

Forward primer

Reverse primer

Fragment size

(bp)

Methylated

GTTGTTATTGGTTAGGAAGTTTTCG

GCGTCTTAAAATAACCACCGTC

114

Unmethylated

TTGTTATTGGTTAGGAAGTTTTTGG

AACACATCTTAAAATAACCACCATC

116

Actb

GGAAATCGTGCGTGACATTA

AGGAAGGAAGGCTGGAAGAG

183

  1. Methylation specific primer (MSP) pairs for Gpx3 used in the methylation specific PCR. The gene Actb was used as control.
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Cancer Cell International

ISSN: 1475-2867

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