Figure 1

Abnormal distribution of nuclear envelope proteins in human cancer cell lines. Immunocytostaining of PC-3, MDA-MB-231, MCF7 and A431 with antibody against Lamin A (A) or emerin (B). Representative images of cells were taken with a fluorescence microscope at 400 × magnification (A) or 600 × magnification (B). Note the invaginations and blebbings pattern of Lamin A and emerin distribution. (C) Immunocytostaining of Lamin A in immortalized and non-malignant NIH 3T3 cells (Mouse embryonic fibroblast cell line) and MCF10A cells (human mammary epithelial cell-line). Representative images of cells were taken with a fluorescence microscope at 600 × magnification.