Figure 2

Existence of progerin in human cancer cell lines. (A) The 12-exon of Lamin A transcript is presented. The cryptic splice site (red box) and the position of the primer set used for RT-PCR is indicated. (B) RT-PCR analyses of progerin transcription in different cancer cells using primers detecting both the full-length (F) and the truncated (T) LMNA isoforms. All PCR products were confirmed by DNA sequencing. (C) Quantitative real time PCR analysis progerin or LMNA from a number of cancer cells (i), a human mammary epithelial cell-line (i), normal and tumor human tissues (ii). Δ150 LMNA RNA was detected by using Dex11-ex12 primers relative to total LMNA RNA detected by using ex8-ex9 primers is shown. (D) Sequencing of progerin and wildtype LMNA RT-PCR product from PC-3 cells. Progerin specific mRNA sequence (GGAGCCCAGAGCCCCCAGA) was compared with the normal LMNA sequence (GGAGCCCAGGTGGGCGGAC). (E) Western blotting of total cell lyses from PC-3, DU145, LNCAP, and MDAMB231 with Lamin A antibody, which detects lamin A/C and progerin simultaneously.