Figure 3

Ectopic progerin expression in cancer cells. NIH-3T3 cells were transfected with different lamin A expression constructs or the vector control. (A) Total cell lysates were probed with GFP antibody for Western blotting. (B) Representative images of cells were taken with a fluorescence microscope at 600 × magnification. Note that, ectopic expression of progerin exacerbated nuclear lamina distribution by inducing nuclear blebbings and invaginations (low panel), indicated by arrows. (C) PC-3 and MCF7 cells were transfected with lamin A expression constructs or vector control. Total cell lysates of PC-3 cells were probed with GFP antibody (i) or GFP and Lamin A/C antibody (ii) for Western blotting. (D) Representative images of cells were taken with a fluorescence microscope at 400 × magnification. (E) The PC-3, MCF7 and NIH-3T3 cells were transfected with Lamin A or progerin expression constructs. The cells were stained with Lamin A antibody. Representative images were obtained with an Olympus Fluoview confocal microscope using a 100× oil immersion objective lens.