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Figure 5 | Cancer Cell International

Figure 5

From: Promotion of tumor development in prostate cancer by progerin

Figure 5

Impaired DNA repairs in PC-3 cells with ectopic progerin expression. PC-3 cells were transfected with different lamin A expression constructs or vector control. The cells were treated with CPT (0.1 μM) for 12 hours and 48 hours later, the cells were stained with either γ-H2AX (A) or Phospho-Chk2 (Thr68) (B) antibody. Representative images of cells were taken with a fluorescence microscope at 400 × magnification. (C) PC-3 cells were transfected with different either progerin-pEGFP or LA-pEGFP as control. The transfected cells were treated with CPT (0.1 μM) with the indicated times. The total cell lysates were probed with either γ-H2AX or Phospho-Chk2 (Thr68) antibody. (D) Representative photomicrographs of the comet assay showing the DNA migration pattern. Representative images of cells were taken with a fluorescence microscope at 600 × magnification. Relative lengths of the DNA tail and Tail moments are presented as below. The bars represent the mean ± SE (n = 20). *P < 0.01; ** P < 0.001, compared with PBS control at the corresponding time. (E) The cells were treated with CPT (0.5 μM) for 12 hours and 48 hours, the cell viability was measured by trypan blue exclusion based cell staining.

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