Figure 6

Elevated tumorigeneis of PC-3 cells with ectopic progerin expression. (A) Five-week growth kinetics of the tumors derived from PC-3 with stable Lamin A, progerin or vector expression. Data point, mean volume of 5 tumors for each group; Data point, mean volume of five tumors; bars, STD. (B) Mitotic index. Mitotic figures were counted in 400× fields and expressed as the percentage of the total cells. Columns, mean mitotic index in tumors; bars, STD. Note the significant increase in mitotic figures in tumors derived from progerin transfectants (n = 5) as compared to those of LA-pEGFP transfectants (n = 5) (p < 0.001 by Student's t test). (C) Apoptotic index. Apoptotic figures were counted in 400× fields and the index expressed as the percentage of the total cells in the same field. Columns, mean apoptotic index in tumors; bars, STD. (p < 0.01 by Student's t test).