Figure 3From: Pancratistatin induces apoptosis in clinical leukemia samples with minimal effect on non-cancerous peripheral blood mononuclear cellsNon-cancerous peripheral blood mono-nucleated cells (PBMCs) are relatively unaffected by pancratistatin. A) Flow cytometry analysis using Annexin-V AlexaFluor 488 was performed on PBMCs that were untreated or treated with 1 μM pancratistatin for 48 h. A minimum of 20 000 events were measured for each sample on a Beckman Coulter Cytomics FC500 flow cytometer. B) Hoechst staining and Annexin-V binding depicts the selective activity of pancratistatin; there is minimal difference in nuclear morphology (i, ii) and amount of externalized phosphatidylserine (iii, iv) between untreated and treated non-cancerous PBMCs.Back to article page