Immunofluorescence based studies of p53BP1 after treatment with chalcone-imidazolone conjugates. MCF-7 Cells were grown in 10% FBS containing medium on cover slips and treated with 30 μM concentration of chalcone imidazolone compounds (6 and 8). TMAC, is the starting material and CA-4, is the positive control used. Cells were fixed in 4% paraformaldehyde and processed for immunofluorescence with p53BP1 antibody as described in materials and methods. Images were captured using confocal microscopy. The pattern of staining changes was also recorded.