Figure 12

mRNA levels of various genes involved in DNA damage at telomeres in MCF-7 cells. MCF-7 cells were treated chalcone-imidazolone conjugates at a final concentration of 30 μM for 24h. After treatment total RNA was isolated and RT-PCR was conducted. The PCR products were separated on 1% agarose gel electrophoresis and visualized under U.V light. GAPDH was used as loading control. Each experiment was repeated three times. The gel pictures shown here were representative of three independent experiments.