Chalcone-imidazolone conjugates cause cell death in MCF-7 cells. Trypan blue exclusion assay showing increase of cell death especially in conjugates 6 and 8. MCF-7 cells were treated at 30 μM concentration of TMAC, CA-4, 6 and 8 for 24 h. Each experiment was conducted for three times. The dead cells have lost the shiny nature and became dark blue coloured. Each experiment was conducted for three times. The p-values was found to be P < 0.001(***) when compared to control non-treated cells.