Figure 4

Immunofluorescence studies to observe apoptotic related morphological changes in chalcone-imidazolone conjugates. MCF-7 cells were grown in DMEM medium with 10% FBS for 12 h. Conjugates (6 and 8) were treated at 30 μM for 24 h time period. The cells were then washed, fixed with 4% paraformaldehyde, and stained with nuclear dye DAPI. The slides were then examined by fluorescence microscopy and photographed. Cells with signs of apoptosis (fragmented nuclei) were observed in chalcone-imidazolone conjugates treated cells, when compared with control, non-treated cells.