Figure 6
From: Chalcone-imidazolone conjugates induce apoptosis through DNA damage pathway by affecting telomeres
Western blot analysis of tumour suppressor proteins (p53 and p21). MCF-7 cells were treated with chalcone-imidazolone conjugates for 24 h. The total protein extracts were prepared after 24 h and analysed with antibodies specific to tumour suppressor proteins such as p53 and p21. β-actin was used as loading control. The The molecular weights of p53, p21 proteins were 53 KDa, 21 KDa respectively.