Effect of chalcone imidazolone conjugates on DNA damage associated proteins. MCF-7 cells treated at 80% confluency with conjugates TMAC, CA-4, 6 and 8 for 24 h. The total cell lysates were harvested and analyzed by immunoblotting with anti-Chk2, anti-Chk1, anti-phospho Chk2T68, anti-phospho Chk1S345 and TRF2. The molecular weights of Chk2, Chk1, Chk2T68, Chk1S345 and TRF2 were 61 KDa, 60 KDa, 61 KDa, 60 KDa and 65 KDa respectively. TMAC is the starting material and CA-4 is positive control. β-actin was used as a loading control. Western blots were representative of three independent experiments. The expression of Chk1, Chk2 and phosphorylated active forms of Chk2T68 and Chk1S345 were found to be up-regulated and levels of telomeric repeat binding factor 2 (TRF2) was found to be down regulated. C represents control untreated cells.