Figure 8

Immunofluorescence based studies of telomere repeat binding factor 2 (TRF2) after treatment with chalcone- imidazolone conjugates. Cells were grown in 10% FBS containing medium on cover slips and treated with 30 μM concentration of chalcone imidazolone compounds (6 and 8). TMAC, is the starting material and CA-4, is the positive control used. Cells were fixed and processed for immunofluorescence as described in materials and methods. Images were captured in confocal microscopy. The pattern of staining changes was also recorded. C represents control untreated cells.