Effects of KA on HUVECs Differentiation. Effect of KA on matrigel tube formation of endothelial cells. Cells treated with (A) 1% ethanol (B) 10 μg/ml (C) 20 μg/ml and (D) 30 μg/ml (E) 40 μg/ml and (F) 100 μg/ml suramin as a positive control (4X). (G) The Dose response relationship of KA on rat aorta assay. The inhibitory effect was found to be in dose response manner. Data was represented as mean ± SD (n = 3). ***P < 0.001.