Skip to main content

Advertisement

Table 2 Hydrogen peroxide (H2O2) is involved in vitamin K3 (VK3) and vitamin C (VC) toxic effect on Jurkat leukemia cells.

From: Vitamin K3 and vitamin C alone or in combination induced apoptosis in leukemia cells by a similar oxidative stress signalling mechanism

Treatment/Assay AO/EB (%) DCF (%)
Untreated < 1 ± 0 2 ± 1
VC (10 mM) 26 ± 2 28 ± 2
VK3 (10 μM) 35 ± 3 39 ± 3
VK3 (10 μM) + VC (10 mM) 48 ± 3 55 ± 3
CP55,940 (10 nM) < 1 ± 0 1 ± 1
CP55,940 (10 nM) + VC (10 mM) 1 ± 1b 1 ± 1b
CP55,940 (10 nM) + VK3 (10 μM) 2 ± 1a 8 ± 2a
CP55,940 (10 nM) + VC (10 mM) + VK3 (10 μM) 3 ± 1c 14 ± 2c
NAC (1 mM) < 1 ± 0 1 ± 0
NAC (1 mM) + VC (10 mM) 2 ± 1b 7 ± 2b
NAC (1 mM) + VK3 (10 μM) 4 ± 1a 12 ± 2a
NAC (1 mM) + VC (10 mM) + VK3 (10 μM) 7 ± 1c 20 ± 2c
  1. Jurkat cells (1 × 106 cell/mL) were left untreated or treated with VC (10 mM) or VK3 (10 μM) alone or with VC (10 mM): VK3 (10 μM) in the absence or presence of the antioxidant CP55,940 (10 nM) and N-acetyl-cysteine (NAC, 1 mM) at 37 °C for 24 h. After this time, nuclear morphological changes and hydrogen peroxide (H2O2) generation were evaluated using acridine orange/ethidium bromide (AO/EB) and 2',7'-dichlorofluorescein diacetate (DCFH2-DA) staining, as described in Material and Methods. Positive apoptotic nuclei and green fluorescent dichlorofluorescein (DCF) positive cells percentage is expressed as mean of percentage (%) ± S.D. from three independent experiments. A one-way ANOVA analysis was performed, p < 0.0001. aSignificantly different from VK3 by Bonferroni post-ho c analysis for DCF and Games-Howell post hoc comparison for AO/EB. bSignificantly different from VC by Bonferroni post-ho c analysis for DCF and Games-Howell post hoc comparison for AO/EB. cSignificantly different from VK3-VC by Bonferroni post-ho c analysis for DCF and Games-Howell post hoc comparison for AO/EB.