RealTime PCR analysis of parental cells and mBMDC/67NR-Hyg clones. Relative gene expression pattern of 67NR-Hyg mouse mammary carcinoma cells, mBMDCs, and mBMDC/67NR-Hyg hybrid cell clones 1-3. Gene expression levels were determined by using the "Mouse Cancer Drug Resistance and Metabolism" RealTime-PCR-array. The expression levels of the appropriate genes of the cancer cell line and mBMDC/67NR-Hyg clones were calculated in relation to the expression level of the appropriate genes of mBMDCs, which were set to 0. Each mBMDC/67NR-Hyg clone exhibits an unique gene expression profile. A difference in the gene expression level of greater than two-fold and less than two-fold was considered as significant.