Figure 6

mBMDC/67NR-Hyg clones exhibit an enhanced resistance towards several chemotherapeutic drugs. XTT proliferation assay was conducted to investigate whether the increased Abcb1a/Abcb1b expression levels of the mBMDC/67NR-Hyg clones were correlated with an enhanced drug resistance. mBMDC/67NR-Hyg clones showed a marked resistance towards 17-DMAG and doxorubicin as well as etoposide and paclitaxel, but not 5-Fluorouracil. Inhibition of Abcb1a/Abcb1b activity by verapamil completely blocked the doxorubicin resistance of mBMDC/67NR-Hyg clones. By contrast, resistance of mBMDC/67NR-Hyg clones towards 17-DMAG, etoposide, and paclitaxel was partially impaired by verapamil indicating that additional ABC transporters or other drug resistance mechanisms are involved in the mBMDC/67NR-Hyg clones resistance towards these chemotherapeutic compounds.