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Figure 5 | Cancer Cell International

Figure 5

From: Different metastasis promotive potency of small G-proteins RalA and RalB in in vivo hamster tumor model

Figure 5

Among three main RalB effectors, PLD1 is essential for HET-SR-RalB high metastatic phenotype. (a) Western blot analysis was used to confirm expression of exogenous RalB G23V effector mutants: ΔN11, D49N and D49E (blocking interaction with PLD1, RalBP1 and exocyst complex respectively). (b) SMA test of HET-SR cells expressing active RalB mutants. Activated RalB as well as its effector domain mutants D49N and D49E give statistically significant increase of SMA compared to the control (empty vector expressing cells) (p < 0,05), while deletion of N-terminus leads to abrogation of activated RalB-dependent SMA stimulation.

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Cancer Cell International

ISSN: 1475-2867

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