Figure 2

Cellular localization of TM601 and Golgin-97 by immunocytochemistry. Immunocytochemistry followed by confocal microscopy showed cellular localization of TM601 (red) in relation to Golgin-97 (green) and nucleus (blue) in human cells: U373 glioma (A, B), U87 glioma (C, D), normal umbilical vascular endothelial HUVEC (E, F), normal astrocytes NHA (G, H, I) and normal fibroblast NHDF (J, K, L) cells. The figure shows a similar Golgi localization of TM601 in gliomas (U373, U87) and HUVEC cells but in NHA and NHDF cells, TM601 is seen away from the trans-Golgi. Shown are data representative of n = 8 experiments. The scale bars on the confocal images = 10 μm, magnification 1890 × (A, C, E, H, I, K, L), 4410 × (B, D, F), 630 × (G, J).