Figure 6

Effect of chlorpromazine and amiloride on uptake of TM601, dextran and transferrin by glioma cells. Images of U373 glioma cells (treated with: dextran-TX at 1 mg/ml (A), dextran-TX at 1 mg/ml in presence of amiloride 300 μM (E), TM601-488 10 μM (B), TM601-488 10 μM in presence of amiloride 300 μM (F), transferrin-TX at 35 μg/ml (C), transferrin-TX at 35 μg/ml in presence of chlorpromazine 10 μg/ml (G), TM601 10 μM (D) and TM601 10 μM in presence of chlorpromazine 10 μg/ml (H). Direct fluorescence is shown in A, B, C, E, F, G and immunocytochemistry in D, H. The level of staining of both controls, dextran and transferrin, were decreased by amiloride or chlorpromazine, respectively. Data are representative of total n = 3 experiments. Scale bars = 10 μm, magnification 1890 ×.