Effect of M344 and cisplatin treatments on the levels of BRCA1 mRNA in our panel of cell lines. BRCA1 mRNA levels were analyzed by RT-PCR following 24 hrs of treatment with 0.5, 1.0, or 5.0 μM M344 either alone or in combination with 2 μg/ml cisplatin. DMSO is the solvent control for the M344 treatment and 2 μg/ml cisplatin is the control for the combination. Values represent the mean +/- SEM of three separate experiments. Statistically significant differences, when present, between 5 μM M344 and DMSO control or 2 μg/ml cisplatin + 5 μM M344 and 2 μg/ml cisplatin alone are indicated by * where P < 0.05, ** where P < 0.01, and *** where P < 0.001.