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Figure 4 | Cancer Cell International

Figure 4

From: Upstream molecular signaling pathways of p27 (Kip1) expression in human breast cancer cells in vitro: differential effects of 4-hydroxytamoxifen and deficiency of either D-(+)-glucose or L-leucine

Figure 4

Effects of tamoxifen, 4-hydroxytamoxifen, and deficiency of D -(+)-glucose, L -leucine, L -methionine or L -cysteine on the phosphorylation of 4E-BP1 in MDA-MB-231 cells. (a and e) Western immunoblot analysis of the effects of D-(+)-glucose deficiency, DMSO, tamoxifen and 4-hydroxytamoxifen on the expression of total 4E-BP1 (eukaryotic translation initiation factor 4E binding protein 1) and (b and e) phosphorylated 4E-BP1. (c and e) Western immunoblot analysis of the effects of the deficiency of L-leucine, L-methionine, or L-cysteine on the expression of total 4E-BP1 and (d and e) phosphorylated 4E-BP1. All assays were performed in triplicates and repeated three times. (f) Correlation between the degree of expression of p27 protein and the degree of down-regulation of the phosphorylated 4E-BP1.

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Cancer Cell International

ISSN: 1475-2867

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