Figure 2

Immunofluorescence studies to observe apoptotic related morphological changes in cells treated with benzothiazole based compounds. HepG2 cells were grown in DMEM medium with 10% FBS for over night time period. Conjugates (YM, A3 and A4) were treated at 4μM for 24 h time period. YM is the standard drug. The cells were then washed, fixed in 4% paraformaldehyde and stained with nuclear dye Hoechst. The slides were then examined by fluorescence microscopy and photographed. Cells with signs of apoptosis (blebbing) were observed in YM, A3, and A4 compound treated cells when compared with untreated cells (C). The p values are derived in compound treated cells and are statistically significant when compared to control. *** indicates p < 0.001.