Figure 5

Western blot analysis of tumour suppressor proteins (p53, PTEN) and PTEN dependent Akt phosphorylation in benzothiazole based compound treated cells. HepG2 cells were treated with benzothiazole based compounds for 24 h and cell lysates were subjected to western blot analysis using antibodies specific to p53, PTEN and Akt Ser 473. β-actin was used as loading control. The molecular weights of p53, PTEN and Akt ser 473 are 53, 47 and 65 KDa. The western blot experiments were repeated three times and densitometric analysis was carried out. The results were statistically significant with p-value <0.001 for A3 and A4 when compared to control.