Figure 7

Effect of benzothiazole based compounds k-Ras and MAPK transcript levels. HepG2 cells were treated with benzothiazole based compounds (YM, A3 and A4) at a final concentration of 4 μM for 24 h. After treatment total RNA was isolated and RT-PCR was conducted. The PCR products were separated on 1% agarose gel electrophoresis and visualized under U.V. light. GAPDH was used as loading control. Each experiment was repeated three times. The gel pictures here were representative of three independent experiments and densitometric analysis was done. The results were found to be statistically significant when compared to control (p-value<0.001).