Figure 3

Changes in expression of apoptosis-related protein in response to treatment with hexane extract and quantification by scanning densitometry of PARP bands intensity. K562 cells were treated with 600, 300 and 150 μg/ml of hexane extract (Hex) for 6, 24 and 48 h. Protein extracts were subjected to western blotting to determinate immunoreactivity of PARP, as described in methods section. PARP 116 KDA and 85KDA bands are shown. Quantification by scanning densitometry of PARP bands intensity (quantified band intensity by computer-assisted densitometric analysis (Densitometer, GS-800, BioRad Quantity One)). A: Untreated cells, B: 60 0 μg/ml, C: 30 0 μg/ml, D: 150 μg/ml, E: 600 μg/ml, F: 300 μg/ml, G: 150 μg/ml, H: 600 μg/ml, I: 300 μg/ml, J: 15 0 μg/ml.